Cytiva Amersham™ ECL™ Western Blotting System

Detect mouse and rabbit primary antibodies on Western blots.  Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting System uses HRP-conjugated secondary antibodies and ECL reagents for detection.

Manufacturer:  Cytiva RPN2108

Catalog No. 45-000-877

Includes: Anti-mouse HRP conjugate, 100μL; anti-rabbit HRP conjugate, 100μL; ECL Detection Reagents 1 and 2, 62.5mL each; Blocking agent, 5g

Description

• Provides rapid, sensitive results using simple protocols
• Designed for use with nitrocellulose and PVDF membranes
• Up to 10 times more sensitive than colorimetric methods
• Results obtained on X-ray film provide hard copy data and avoid fading associated with colorimetric detection methods
• Membranes can be reprobed up to 10 times
• Linear response in the range 0.2 to 2 O.D. units using pre-flashed Hyperfilm™ ECL
• Non-radioactive

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Specifications

• Quantity: Each
• For Use With (Application): Detect mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents
• Kit Contents: 100μL Anti-Mouse HRP conjugate, 100μL Anti-Rabbit HRP conjugate, 62.5mL ECL™ detection reagent 1, 62.5mL ECL™ detection reagent 2, 25g blocking agent, sufficient for 10 blots (10 x 10)