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Cytiva Amersham™ ECL™ Western Blotting System

Detect mouse and rabbit primary antibodies on Western blots.  Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting System uses HRP-conjugated secondary antibodies and ECL reagents for detection.

Supplier:  Cytiva RPN2108

Catalog No. 45-000-877

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Includes: Anti-mouse HRP conjugate, 100μL; anti-rabbit HRP conjugate, 100μL; ECL Detection Reagents 1 and 2, 62.5mL each; Blocking agent, 5g



  • Provides rapid, sensitive results using simple protocols
  • Designed for use with nitrocellulose and PVDF membranes
  • Up to 10 times more sensitive than colorimetric methods
  • Results obtained on X-ray film provide hard copy data and avoid fading associated with colorimetric detection methods
  • Membranes can be reprobed up to 10 times
  • Linear response in the range 0.2 to 2 O.D. units using pre-flashed Hyperfilm™ ECL
  • Non-radioactive



Western Blot
100μL Anti-Mouse HRP conjugate, 100μL Anti-Rabbit HRP conjugate, 62.5mL ECL™ detection reagent 1, 62.5mL ECL™ detection reagent 2, 25g blocking agent, sufficient for 10 blots (10 x 10)
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