Aminopeptidase N/CD13 Antibody (APN/514), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24468200	Novus Biologicals NBP2446820.02MG	0.02 mg
NBP24468201	Novus Biologicals NBP2446820.1MG	0.1 mg
NBP24468202	Novus Biologicals NBP2446820.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Aminopeptidase N/CD13 Monoclonal specifically detects Aminopeptidase N/CD13 in Human samples. It is validated for ELISA.

Specifications

• Antigen: Aminopeptidase N/CD13
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, AP-M, AP-N, CD13 antigen, CD13APN, EC 3.4.11, EC 3.4.11.2, gp150, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, P150, PEPNhAPN
• Gene Symbols: ANPEP
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb recognizes an extracellular epitope of an integral membrane glycoprotein of 150kDa, identified as CD13. This antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. It is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.

• Clone: APN/514
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P15144, P15144
• Gene ID (Entrez): 290
• Immunogen: Recombinant human CD13 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 150 kDa

For Research Use Only