Aminopeptidase N/CD13 Mouse anti-Human, Clone: B-F10, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247891B	Novus Biologicals NBP2478910.2MG	0.2 mg

Description

Aminopeptidase N/CD13 Monoclonal specifically detects Aminopeptidase N/CD13 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Aminopeptidase N/CD13
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Myeloid derived Suppressor Cell, Stem Cell Markers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 290
• Immunogen: KG-1 myeloid cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 150 kDa

• Clone: B-F10
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, AP-M, AP-N, CD13 antigen, CD13APN, EC 3.4.11, EC 3.4.11.2, gp150, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, P150, PEPNhAPN
• Gene Symbols: ANPEP
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N). The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.