Invitrogen™ Amphiphysin Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA518513

Catalog No. PIPA518513

Description

This antibody is predicted to react with canine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

Specifications

• Antigen: Amphiphysin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Amph
• Gene Alias: Amph; AMPH1; amphiphysin; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin 1; amphiphysin I; HGNC:471
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 218038, 273
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P49418, Q7TQF7
• Gene Symbols: Amph
• Immunogen: Synthetic peptide sequence (KRKDESRISKAE) corresponding to the internal amino acids of AMPH (aa 159-170).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG