Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. PeproTech Amphiregulin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™ 

PeproTech Amphiregulin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. 500P322BT50
Encompass_Preferred
Change view
Click to view available options
Quantity:
25 μg
50 μg
500 μg
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
500P322BT50 50 μg
500P322BT25 25 μg
50-271-8509 500 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
This item is not returnable. View return policy
Catalog No. 500P322BT50 Supplier PeproTech Supplier No. 500P322BT50UG
Add to Cart
Add to Cart
This item is not returnable. View return policy

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Amphiregulin. Western Blot: To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P322BT-1MG will be provided as 2 x 500 μg

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Amphiregulin
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene AREG
Gene Accession No. P15514
Gene Alias amphiregulin; amphiregulin B; AR; AREG; AREGB; colorectum cell-derived growth factor; CRDGF; Mcub; MGC13647; schwannoma-derived growth factor; SDGF
Gene Symbols AREG
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human Amphiregulin.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 374
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.