Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

Catalog No.	Quantity
43-118-06	250 Units
43-118-16	1000 Units
43-118-20	3000 Units
43-118-18	5000 Units
43-177-42	25,000 Units

Catalog No. 43-118-06 Supplier Applied Biosystems™ Supplier No. 4311806

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Features

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Gold Buffer with MgCl₂

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.

GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

Notes

• AmpliTaq Gold 360 DNA Polymerase is available for even better PCR performance.
• AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL
  • GeneAmp 10X Gold Buffer, 1 x 1.5 mL
  • 25 mM MgCl₂, 1 x 1.5 mL
  
  Store at -15°C to -30°C.
• Format: Tube
• GC-Rich PCR Performance: Low
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Quantity: 250 Units
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• Hot Start: Built-In Hot Start
• No. of Reactions: 200 Reactions
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 5 kb or less
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.