Invitrogen™ Human Angiostatin (Plasminogen) ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
EHANGIOSTAT	Invitrogen™ EHANGIOSTATIN	96 Tests
50-274-1177	Invitrogen™ EHANGIOSTATINX10	10 x 96 Tests
50-255-0895	Invitrogen™ EHANGIOSTATINX5	5 x 96 Tests

Description

The Human Angiostatin (Plasminogen) ELISA quantitates Hu Angiostatin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Angiostatin. Principle of the method The Human Angiostatin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P00747
• Assay Sensitivity: 20 ng/mL
• Product Type: ELISA
• Target Species: Human
• Gene ID (Entrez): 5340
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 31.25 to 2000 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PLG
• Intraassay CV: <10
• Label or Dye: HRP
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Target: Angiostatin
• Total Assay Time: 4 hrs 45 mins