Invitrogen™ Ankyrin 1 Monoclonal Antibody (N388A/10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527723

Catalog No. PIMA527723

Description

A 1:100 dilution of MA5-27723 was sufficient for detection of Ankyrin R in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G. This antibody was formerly sold as clone S388A-10.

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Specifications

• Antigen: Ankyrin 1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene Accession No.: P16157, Q02357
• Gene Symbols: ANK1
• Immunogen: Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Clone: N388A/10
• Conjugate: Unconjugated
• Gene: ANK1
• Gene Alias: ANK; ANK1; ANK-1; ANK-1; erythrocyte skeletal protein; ankyrin 1; ankyrin 1, erythrocytic; ankyrin 1, erythroid; ankyrin-1; Ankyrin-R; erythrocyte ankyrin; nb; normoblastic anemia; pale; rCG_43073; SPH1; SPH2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 11733, 286, 306570
• Content And Storage: -20°C
• Form: Liquid