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Invitrogen™ Annexin V Recombinant Protein, FITC, eBioscience™

Catalog No. 509289
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Quantity:
100 Tests
20 Tests
300 Tests

Recombinant Protein

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. Description: Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Purity: Annexin V is >89% pure as demonstrated by SDS-gel electrophoresis and reverse phase HPLC. FITC labelling allows simple direct detection of PS by FACS analysis. Counterstaining by propidium iodide allows the discrimination of apoptotic cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P08758, P14668, P48036
Concentration 5μL/Test
For Use With (Application) Control, Flow Cytometry
Formulation TBS with 1% BSA and 0.02% sodium azide; pH 7.4
Gene ID (Entrez) 308
Molecular Weight (g/mol) 35.8kDa
Name Annexin V
pH Range 7.4
Purification Method SDS-gel electrophoresis and reverse phase HPLC
Quantity 20 Tests
Source E. coli
Storage Requirements 4°C, do not freeze
Regulatory Status RUO
Structural Form E. Coli
Common Name Annexin V
Gene Symbol Anxa5
Biological Activity Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Product Type Recombinant Protein
Specific Reactivity All
Conjugate FITC
Cross Reactivity All
Species E. coli
Recombinant Recombinant
Form Liquid
Protein Subtype Recombinant
Purity or Quality Grade Affinity chromatography
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WARNING: Cancer - www.P65Warnings.ca.gov
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