MilliporeSigma™ anti-ACTN4 Clone: 4D10,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ CB1024100UG

Catalog No. CB1024100UG

Description

Specifically detects ACTN4 Clone: 4D10 in Human, Mouse, Rat samples, and it is validated for ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immuno Blotting

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Specifications

• Antigen: ACTN4
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: ELISA, Immunoblot, Immunoblot, Immunocytochemistry, Immunohistochemistry (Paraffin)
• Clone: 4D10
• Formulation: In PBS, pH 7.2.
• Immunogen: A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽90-100kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
• Content And Storage: −20°C
• Isotype: IgG2a

For Research Use Only