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AFAP Mouse, Unlabeled, Clone: 36, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610199

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Catalog No. BDB610199


Description

Description

Chicken embryo fibroblasts (CEF) which are transformed by pp60[src] show anincrease in the steady-state levels of tyrosine-phosphorylated proteins. One of these proteins is the Actin Filament-Associated Protein, AFAP110. This protein forms a stable complex with activated pp60[src] via the SH3 and SH2 domains ofthe src protein. However, tyrosine phosphorylation of AFAP requires only the intact src SH3 domain. AFAP is associated with actin stress fibers in normal CE cells, but following transformation by pp60[src], AFAP becomes localized in rosette-like structures known as podosomes. Several sequence motifs within AFAP110 are predicted to bind to SH2 and to SH3 domains. In vitro analysis of AFAP in normal cell extracts demonstrated that it binds less efficiently to Src SH2-containing fusion proteins than does AFAP from src-transformed cell extracts.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

AFAP
Monoclonal
250μg/mL
Chicken, Human, Mouse, Rat
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
50 μg
Cell Biology
Chicken, Human, Mouse, Rat
Immunofluorescence, Immunohistochemistry, Western Blot
36
Unconjugated
Mouse
Affinity Purified
RUO
Primary
IgG1
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