MilliporeSigma™ anti-Akt, PH Domain Clone: SKB1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ ST10881SET

Catalog No. ST10881SET

Description

Specifically detects Akt, PH Domain Clone: SKB1 in Human, Mouse, Rat samples, and it is validated for Flow Cytometry, Immunoprecipitation, Immuno Blotting

Akt (Accession No. NM_005163) is primarily a cytosolic enzyme that acts as a downstream effector of phosphatidylinositol 3-kinase (PI-3K) activation. Akt has been shown to be activated by a variety of stimuli, including hematopoetic cytokines, various chemokines, heat shock, hypoxia,hyperosmolarity, integrins, nerve growth factor (NGF), platelet- derived growth factor (PDGF), and T cell receptor signaling. Suggested to play a role in regulating the balance between cellular mitogenesis and apoptosis. Akt has been shown to be constitutively active in some human cancers.

Specifications

• Antigen: Akt, PH Domain
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 1 Set
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunoblot, Immunoblot, Immunoprecipitation
• Clone: SKB1
• Formulation: 100μg antibody in 150mM NaCl, 100mM Tris-glycine, 30% glycerol, pH 7.4 and 100μg control lysate.
• Immunogen: a recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽60kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit. Antibody Target Gene Symbol: AKT1 Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1 Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
• Content And Storage: −20°C
• Isotype: IgG

For Research Use Only