Akt1 Mouse, Alexa Fluor 647, Clone: 55/PKBa/Akt, BD

Catalog No.	Quantity
BDB560050	50 Tests

Catalog No. BDB560050 Supplier BD Biosciences Supplier No. 560050

Description

Mouse Monoclonal Antibody

Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases] is a family of serine/threonine kinases that contains a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinase products bind to the PH domain, resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473. Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (Bad). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors.

The 55/PKBa/Akt monoclonal antibody recognizes Akt1, regardless of phosphorylation status.

The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.

Specifications

• Antigen: Akt1
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 55/PKBa/Akt
• Conjugate: Alexa Fluor 647
• Description: PKBα, RAC-PK
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Akt1 Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Rat
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ