Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. PC284100UG

MilliporeSigma™ anti-AML1 (Ab-1), Polyclonal

Catalog No. PC284100UG Shop All MilliporeSigma Products
Change view
Click to view available options
Quantity:
100 μg
Conjugate:
Unconjugated
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Conjugate
PC284100UG 100 μg Unconjugated
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PC284100UG Supplier MilliporeSigma™ Supplier No. PC284100UG

Description

Rabbit Polyclonal Antibody

Specifically detects AML1 Clone: in Human, Mouse samples, and it is validated for Gel Supershift, Immunoprecipitation, Immuno Blotting

The family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of the hematopoietic lineage and the bone cell lineage. There are currently three different mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFbeta and it is this complex which has the transcription factor activity. Within the complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus and has homology with the Drosophila Runt gene that is the DNA binding protein of the protein. In leukemias, AML1 undergoes a translocation and becomes fused to MTG8 (ETO) and forms a chimeric protein (83kDa). This chimera is in turn complexed with the CBFbeta gene product that also has undergone a translocation. In addition to MTG8, AML1 has also been shown to undergo translocation with TEL, and EVI. Splice variants have been reported for AML which include AML1a (30kDa), AML1b (54kDa), and AML1c. All forms contain the runt domain but vary in their transactivation ability. Additionally 6 major transcripts have been shown for AML due to the use of 2 promoters and differential use of 3 polyadenylation sites in addition to the splice variants.
TRUSTED_SUSTAINABILITY

Specifications

Antigen AML1
Applications Gel Supershift, Immunoblot, Immunoblot, Immunoprecipitation
Classification Polyclonal
Conjugate Unconjugated
Formulation In PBS.
Host Species Rabbit
Immunogen a synthetic peptide corresponding to amino acids at the N-terminus of human AML1
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Recognizes the ∽58kDa AML1 protein in HeLa, Jurkat, and NIH2T3 cells. May not detect endogenous AML1.
Target Species Human, Mouse
Content And Storage −20°C
Form Liquid
Isotype IgG
Show More Show Less

For Research Use Only

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.