MilliporeSigma anti-AML3, Polyclonal
Rabbit Polyclonal Antibody
Manufacturer: MilliporeSigma PC287100UG
Specifically detects AML3 Clone: in Human, Mouse, Rat samples, and it is validated for Gel Supershift, Immuno BlottingThe family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of hematopoietic and bone cell lineage. There are currently three mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFβ and it is this complex that produces the transcription factor activity. Within this complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus is homologous with theDrosophila runt gene that acts as the DNA binding area of the protein. AML3, the third member of the AML family, is unique from the other family members in that it also possesses osteoblast-specific transcriptional regulation properties. AML3 has been demonstrated as a single transcript of 5.4 kb in bone tissues where the protein encoded by the gene has been shown to be present in the osteoblast-specific promoter binding complex. Like other AML proteins, AML3 contains the runt domain, a putative ATP binding site, and a proline and serine rich C-terminal half. AML3 is also the predominant runt domain containing transcription factor in Buffalo Rat liver cells.
|a synthetic peptide [(K)TDVPRRISDDDTATSD] corresponding to amino acids 319-334 from human AML3|
|Human, Murine, Rat|
|Gel Supershift, Immuno Blotting, Immunoblot|
|Recognizes the ∽65kDa, the ∽60kDa, and the ∽45kDa AML3 proteins in transfected COS or BRL (buffalo rat liver) cells.|
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