Invitrogen™ Amphiregulin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527298

Catalog No. PIPA527298

Description

Recommended positive controls: AREG-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

Specifications

• Antigen: Amphiregulin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AREG
• Gene Alias: amphiregulin; amphiregulin B; AR; AREG; AREGB; colorectum cell-derived growth factor; CRDGF; Mcub; MGC13647; schwannoma-derived growth factor; SDGF
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 374
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.17 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P15514
• Gene Symbols: AREG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Amphiregulin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG