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Apaf-1 Mouse anti-Human, Unlabeled, Clone: 24, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 611365

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Catalog No. BDB611365

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than “free” caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis.

Immunofluorescence, Western Blotting

Specifications

Specifications

Apaf-1
24
Unconjugated
Murine
IgG1
RUO
Primary
Human
Immunofluorescence, Western Blot
250μg/mL
Affinity Purified
Human Apaf-1 aa. 252-445
150μg
Cell Biology
Monoclonal
Documents
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