MilliporeSigma™ anti-APC Clone: FE9,

Catalog No.	Quantity	Conjugate
OP44100UG	100 μg	Unconjugated

Catalog No. OP44100UG Supplier MilliporeSigma™ Supplier No. OP44100UG

Description

Mouse Monoclonal Antibody

Specifically detects APC Clone: FE9 in Human, Mouse, Rat samples, and it is validated for Immuno Blotting

Colon cancer generally occurs sporadically although there is clear genetic predisposition in some families. Familial Adenomatous Polyposis Coli (FAP) is an autosomal dominantly inherited disorder that exhibits 80 to 100% penetrance and predisposes to colon cancer. The gene responsible for FAP has been assigned to chromosome 5q21, a region that is often lost in tumors from patients with sporadic colorectal cancer. Recently, a gene called APC, for adenomatous polyposis coli, from the 5q21 locus was isolated and shown to contain mutations in DNA prepared from tumors of patients with sporadic colorectal cancer and in germ line DNA and tumor DNA from FAP patients. In 79 unrelated patients with FAP, mutations in APC were found in 67% of the cases; and 49 of the 53 mutations were either small deletions/insertion or were point mutations that introduced stop codons into the reading frame. Furthermore, APC mutations have been found in the earliest tumors that could be analyzed (0.5 cm diameter) and the mutation frequency remained constant as tumors progressed from benign to malignant stages. Analysis of the APC protein demonstrates that inactivating mutations result in stable truncated proteins that can be identified in colon tumors from both FAP and sporadic colon cancer patients as well as in constitutional cells of FAP patients. Loss of heterozygosity at the APC locus has also been reported for esophageal cancer and lung cancer.

Specifications

• Antigen: APC
• Applications: Immunoblot
• Classification: Monoclonal
• Clone: FE9
• Conjugate: Unconjugated
• Formulation: In 50mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.
• Host Species: Mouse
• Immunogen: a synthetic peptide corresponding to the N-terminal 35 amino acids of APC
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. Antibody Target Gene Symbol: APC Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC Entrez Gene Name: adenomatous polyposis coli
• Target Species: Human, Mouse, Rat
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG1

For Research Use Only