MilliporeSigma™ anti-ApoE4 Fragment nApoECF, Polyclonal

Catalog No.	Quantity	Conjugate
ABN1350MI	100 μL	Unconjugated

Catalog No. ABN1350MI Supplier MilliporeSigma™ Supplier No. ABN1350

Description

Rabbit Polyclonal Antibody

Specifically detects ApoE4 Fragment nApoECF Clone: in Human samples, and it is validated for Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting

Apolipoprotein E (UniProt P02649; also known as Apo-E) is encoded by the APOE (also known as AD2, LDLCQ5, LPG) gene (Gene ID 348) in human. Apolipoprotein E (ApoE) is found in the chylomicron and Intermediate-density lipoprotein (IDLs) and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. ApoE is initially produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the 299 a.a. mature protein. Three additional natural variants (E2, E3, and E4) exist due to polymorphisms. E4 has an allele frequency of approximately 14 percent and has been implicated in atherosclerosis and various CNS disorders, including Alzheimer's disease (AD). ApoE4 is highly susceptible to proteolysis, which impairs its role in cholesterol transport and beta-amyloid removal in the CNS. Various ApoE4 fragments (14–20kDa) have been identified in the AD brain. The C-terminal portion of apoE has been implicated in binding to beta-amyloid and is localized to plaques, while the N-terminal domain preferentially localizes within neurofibrillary tangles (NFTs) in the AD brain.

Specifications

• Antigen: ApoE4 Fragment nApoECF
• Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: P02649
• Gene Symbols: APOE
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to the C-terminal sequence of human ApoE4 fragment nApoECF (Asp172).
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000032
• Test Specificity: Specifically recognizes the ApoE4 N-terminal fragment(s) generated by a proteolytic cleavage between Asp172 and Leu173 of ApoE4. Does not recognize full-length ApoE3 and ApoE4, nor ApoE4 fragments with C-termi other than D172.
• Target Species: Human
• Content And Storage: 2°C to 8°C for one year

For Research Use Only