MilliporeSigma™ anti-APP A4, a.a. 66-81 of APP {NT} Alexa Fluor 647, Clone: 22C11,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MAB348AF647

Catalog No. MAB348AF647

Description

Specifically detects APP A4, a.a. 66-81 of APP {NT} Clone: 22C11 in Canine, Fish, Human, Monkey, Mouse, Porcine, Rat samples, and it is validated for Immunocytochemistry

Amyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. Amyloid precursor protein (APP) is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature protein with a large extracellular portion (a.a. 18-699), followed by a transmembrane segment (a.a. 700-723) and a cytoplasmic (a.a. 724-770) tail. APP can be further processed by the α-, β-, and γ-secretases in two alternative processing pathways. In the non-amyloidogenic pathway, APP is first cleaved by the plasma membrane-localized α-secretase to generate an N-terminal extracellular sAPPα fragment (a.a. 18-687) and a membrane-bound C-terminal fragment C83 (CTFα), which can be further cleaved by γ-secretase to produce a non-toxic small peptide p3 and a cytoplasmic APP intracellular domain (AICD). In the amyloidogenic pathway, APP undergoes β-cleavage in BACE-1 (β-site APP-cleaving enzyme)-enriched endosomes to generate an N-terminal extracellular sAPPβ fragment (a.a. 18-671) and a membrane-bound C-terminal fragment C99 (CTFβ). Subsequent cleavage of C99 by γ-secretase releases the amyloid β peptide (Aβ) and AICD. Aβ accumulation in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer's disease (AD).

Specifications

• Antigen: APP A4, a.a. 66-81 of APP {NT}
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Gene Accession No.: P05067
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 22C11 reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich, C., et al. (1993). J. Biol. Chem. 268(35):26571-26577). Clone 22C11 recognizes all three isoforms of APP, immature ∽110kDa, sAPP ∽120kDa, and mature ∽130kDa (Hoffmann, J., et al. (2000). Eur. J. Cell. Biol.79(12):905-914.). The antibody is known to cross react with APLP2 (Slunt, H.H., et al. (1994). J. Biol. Chem. 269(4):2637-2644).
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1

• Applications: Immunocytochemistry
• Clone: 22C11
• Formulation: Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
• Gene Symbols: APP, A4, AD1
• Immunogen: Purified recombinant Alzheimer precursor A4 (pre-A4695) fusion protein.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NM_201414, NM_000484.2, NM_201413.1
• Target Species: Canine, Fish, Human, Monkey, Mouse, Pig, Rat
• Form: Purified

For Research Use Only