MilliporeSigma™ anti-ATP Synthase subunit beta Clone: 11/21-7-A8,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1304

Catalog No. MABS1304MI

Description

Specifically detects ATP Synthase subunit β Clone: 11/21-7-A8 in Human, Mouse, Rat samples, and it is validated for Dot Blot, ELISA, Immunocytochemistry, Western Blotting

ATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria.

Specifications

• Antigen: ATP Synthase subunit β
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: P06576
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Dot Blot, ELISA, Immunocytochemistry, Western Blot
• Clone: 11/21-7-A8
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: ATP5B, ATPMB, ATPSB
• Immunogen: His-tagged recombinant protein corresponding to human ATP Synthase subunit β.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001677
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1 κ

For Research Use Only