MilliporeSigma™ anti-Bak Clone: TC-100,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ AM03100UG

Catalog No. AM03100UG

Description

Specifically detects Bak Clone: TC-100 in Mouse, Rat samples, and it is validated for Immunoblotting, Immunocytochemistry, Western Blotting

Bak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. Bak was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The Bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4kDa but migrates by gel electrophoresis as an approximately 30kDa protein under denaturing conditions. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner, has been found in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.

Specifications

• Antigen: Bak
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: −20°C
• Isotype: IgG2a

• Applications: Immunoblot, Immunocytochemistry, Western Blot
• Clone: TC-100
• Formulation: In 0.05M sodium phosphate buffer, 0.2% gelatin.
• Immunogen: recombinant human Bak from which the C-terminal and transmembrane domains have been deleted
• Regulatory Status: RUO
• Target Species: Mouse, Rat
• Form: Purified

For Research Use Only