Bcl-2 Hamster anti-Mouse, FITC, Clone: 3F11, BD

Catalog No.	Quantity
BDB554221	100 Tests

Catalog No. BDB554221 Supplier BD Biosciences Supplier No. 554221

Description

Includes

FITC Hamster Anti-Mouse Bcl-2 and FITC Hamster IgG Isotype Control (Anti-Trinitrophenol (TNP))

Hamster Monoclonal Antibody

Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the cellular response to apoptotic signals. Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types. Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kD intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.

Clone 3F11 reacts with mouse Bcl-2. It does not cross-react with human Bcl-2. Gel purified recombinant mouse Bcl-2 protein was used as immunogen.

Clone A19-3 is an Armenian hamster IgG isotype control which is specific for the hapten trinitrophenol (TNP). This hapten is not expressed on human cells or human cell lines. The solutions are free of unconjugated antibody. The 3F11 and A19-3 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.

Specifications

• Antigen: Bcl-2
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 3F11
• Conjugate: FITC
• Host Species: Hamster
• Immunogen: Recombinant Mouse Bcl-2, TNP-keyhole limpet hemocyanin
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.