MilliporeSigma™ anti-beta Actin, Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABT264

Catalog No. ABT264MI

Description

Specifically detects beta Actin Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting

Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase).β- andγ-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, theβ-, but not theγ-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylatedβ-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.

Specifications

• Antigen: beta Actin
• Classification: Polyclonal
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: ACTB
• Immunogen: C-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification.
• Quantity: 100 μg
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_001092
• Target Species: Human, Mouse, Rat

• Applications: Immunocytochemistry, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P60709
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal antibody detects beta-actin posttranslationally modified by the removal of Met1 and Asp 2 and the arginylation of Asp3. This antibody does not react with beta-actin with unmodified N-terminus.
• Content And Storage: 2°C to 8°C for one year

For Research Use Only