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MilliporeSigma™ anti-beta Actin, Polyclonal
Rabbit Polyclonal Antibody
Supplier: MilliporeSigma™ ABT264
Description
Specifically detects beta Actin Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting
Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase).β- andγ-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, theβ-, but not theγ-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylatedβ-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.
Specifications
beta Actin | |
Polyclonal | |
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide. | |
ACTB | |
C-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification. | |
100 μg | |
Cell Structure | |
NP_001092 | |
Human, Mouse, Rat |
Immunocytochemistry, Western Blot | |
Unconjugated | |
P60709 | |
Rabbit | |
Affinity Purified | |
RUO | |
Primary | |
This polyclonal antibody detects beta-actin posttranslationally modified by the removal of Met1 and Asp 2 and the arginylation of Asp3. This antibody does not react with beta-actin with unmodified N-terminus. | |
2°C to 8°C for one year |
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