Bid Mouse anti-Human, Unlabeled, Clone: 7, BD

Catalog No.	Quantity
BDB611528	50 μg

Catalog No. BDB611528 Supplier BD Biosciences Supplier No. 611528

Description

Mouse Monoclonal Antibody

Members of the Bcl-2 protein family function to inhibit (Bcl-2, Bcl-XL, Mcl-1, A1) or promote (Bax, Bak, Bcl-Ls, Bad) apoptosis. Bid is a Bcl-2 family death agonist that heterodimerizes with either agonists (Bax) or antagonists (Bcl-2). Bid contains a centrally located BH3 domain that allows interaction with the BH1 domain of Bax and Bcl-2. However, Bid does not contain other domains commonly found in Bcl-2 family members, such as BH1, BH2, or BH4. In addition, Bid does not contain a C-terminal hydrophobic region that is characteristic of membrane-bound Bcl-2 family members. Singlet oxygen-induced apoptosis in human leukemia cells and Fas-induced apoptosis involve caspase-8 cleavage of Bid, which produces a 15 kDa C-terminal fragment and a 6.5 kDa N-terminal fragment. The C-terminal fragment translocates to the mitochondria and promotes the release of cytochrome C through a mechanism that might involve destabilization of the mitochondrial membrane. Thus, the proapoptotic role of Bid may involve mitochondrial membrane destabilization, as well as multiple protein-protein interactions.

Specifications

• Antigen: Bid
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 7
• Concentration: 250μg/mL
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Mouse Bid aa. 50-62
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at -20°C.
• Isotype: IgG1