MilliporeSigma™ anti-BRCA2, Polyclonal

Catalog No.	Quantity	Conjugate
CA1033100UG	100 μg	Unconjugated

Catalog No. CA1033100UG Supplier MilliporeSigma™ Supplier No. CA1033100UG

Description

Rabbit Polyclonal Antibody

Specifically detects BRCA2 Clone: in Human samples, and it is validated for Immunohistochemistry (Paraffin), Immunoprecipitation

Inherited mutations in the BRCA2 gene enhances the risk of familial breast cancer with the neoplasia related to loss of the wild type allele. Human BRCA2 encodes a 3418 amino acid protein with little homology to any known proteins. Transcription of BRCA2 appears to be generally co-ordinately regulated and co-locally expressed with BRCA1 during proliferation and differentiation with low level expression in early G1 and maximal expression occurring during the G1/S transition of the cell cycle. However, BRCA1 and BRCA2 expression is differentially regulated during the development of specific endocrine tissues suggesting a gene-specific response to sex hormones. While loss of BRCA2 is associated with early onset breast cancer, the gene is critical for embryogenesis since nullizygous mice with a deletion of the 5' region of exon 11 arrest development by day 8.5 of gestation. Recently, however, mice carrying homozygous inactivating mutations due to deletion of exon 11 sequences or insertion of selectable markers in the 3' portion of the cDNA have been obtained, albeit with severe growth characteristics including lack of germinal cells, small stature, and skeletal deformities. Loss of BRCA2 expression in embryogenesis does not appear to be due to apoptosis, but proliferation is impaired, perhaps due to enhanced expression of p21WAF1. Although the precise role for BRCA2 is still to be determined, recent studies implicate BRCA2 in DNA repair and recombination processes partly due to association with the Rad51 protein.

Specifications

• Antigen: BRCA2
• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: In PBS.
• Host Species: Rabbit
• Immunogen: a recombinant protein consisting of amino acids 3245-3418 of human BRCA2
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽400kDa BRCA2 protein in MCF-7 and HeLa cells and breast carcinoma.
• Target Species: Human
• Content And Storage: −20°C
• Form: Liquid
• Isotype: IgG

For Research Use Only