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MilliporeSigma™ anti-BrdU Clone: Mobu-1,

Catalog No. NA61100UG Shop All MilliporeSigma Products
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Quantity:
100 μg
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
NA61100UG 100 μg Unconjugated
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Catalog No. NA61100UG Supplier MilliporeSigma™ Supplier No. NA61100UG
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Mouse Monoclonal Antibody

Specifically detects BrdU Clone: Mobu-1 in Multi samples, and it is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Proliferating cells are usually detected by incorporation of [3H]-thymidine into DNA and quantitation by autoradiography or scintillation counting. Alternatively, bromodeoxyuridine (BrdU), an analog of thymidine, can be incorporated specifically into the DNA of replicating cells and the extent of incorporation can be detected using antibodies directed against the incorporated analog. The proportion of cells actively proliferating can then be determined by immunofluorescence or flow cytometry as well as immunohistochemistry (which has been done with tumors, tissues, and blood cell elements).
TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Applications Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone Mobu-1
Conjugate Unconjugated
Formulation In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Host Species Mouse
Immunogen BrdU-labeled DNA
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Recognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
Target Species Multi-species
Content And Storage 2°C to 8°C
Form Purified
Isotype IgG1
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