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MilliporeSigma™ anti-BRE1-A (RNF20) Clone: 8A4.2,

Catalog No. MABE948MI Shop All MilliporeSigma Products
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Quantity:
100 μg
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
MABE948MI 100 μg Unconjugated
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Catalog No. MABE948MI Supplier MilliporeSigma™ Supplier No. MABE948
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Description

Mouse Monoclonal Antibody

Specifically detects BRE1-A (RNF20) Clone: 8A4.2 in Human samples, and it is validated for Western Blotting

E3 ubiquitin-protein ligase BRE1A (UniProt Q5VTR2; also known as BRE1 E3 ubiquitin ligase homolog, BRE1-A, hBRE1, Homolog of S. cerevisiae BRE1, RING finger protein 20) is encoded by the RNF20 (also known as BRE1, BRE1A) gene (Gene ID 56254) in human. BRE1A (RNF20) is a RING finger domain-containing protein originally identified as the E3 uqibiquitin ligase that catalyzes histone H2B Lys120 monoubiquitination in yeast. The histone chaperone FACT is reported to mediate RNF20 recruitment for chromatin remodeling to initiate homologous recombination repair (HRR). RNF20 is also shown to negatively regulate hepatic fatty acid metabolism by targeting SREBP1c for ubiquitination and subsequent proteasomal degradation. In addition, RNF20 is shown to mediated the polyubiquitination of ErbB3-binding protein 1 (Ebp1) and Transcription factor activator protein 2α (AP-2α). AP-2α functions as a negative regulator of adipogenesis by suppressing the transcription of CCAAT/enhancer binding protein (C/EBPα). Overexpression of RNF20 in 3T3-L1 cells results in a down-regulation of endogenous AP-2α and a concomitant transcriptional upregulation of C/EBPα.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BRE1-A (RNF20)
Applications Western Blot
Classification Monoclonal
Clone 8A4.2
Conjugate Unconjugated
Formulation Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No. Q5VTR2
Gene Symbols RNF20, BRE1, BRE1A
Host Species Mouse
Immunogen GST-tagged recombinant protein corresponding to the third coiled-coil region of human BRE1-A (RNF20).
Purification Method Protein G purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Epigenetics, Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_062538
Target Species Human
Content And Storage 2°C to 8°C for one year from date of shipment
Form Purified
Isotype IgG2a κ
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For Research Use Only

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