MilliporeSigma™ anti-c-H-Ras Clone: F235-1.7.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ OP23100UG

Catalog No. OP23100UG

Description

Specifically detects c-H-Ras Clone: F235-1.7.1 in Human, Mouse, Rat samples, and it is validated for Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting

The human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.

Specifications

• Antigen: c-H-Ras
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
• Clone: F235-1.7.1
• Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
• Immunogen: recombinant p21 Ras
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽21kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
• Content And Storage: 2°C to 8°C
• Isotype: IgG1

For Research Use Only