Calcineurin Mouse anti-Human, Unlabeled, Clone: G182-1847, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556350

Catalog No. BDB556350

Description

Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (∽61 kDa) is catalytic and has calmodulinbinding activity; the B subunit (∽19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.

G182-1847 recognizes the α and β isoforms of the A subunit of calcineurin (∼61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human β1 isoform of calcineurin A subunit was used as immunogen.

Specifications

• Antigen: Calcineurin
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human β1 isoform of calcineurin A Subunit aa. 349-505
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

• Applications: Western Blot
• Clone: G182-1847
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.