MilliporeSigma™ anti-Caspase 3, Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABC495

Catalog No. ABC495MI

Description

Specifically detects Caspase 3 Clone: in Human, Mouse samples, and it is validated for Immunocytochemistry, Western Blotting

Caspase-3 (EC 3.4.22.56; UniProt P42574; also known as Apopain, CASP-3, CPP-32, PARP cleavage protease, SCA-1, SREBP cleavage activity 1, Caspase 3 apoptosis-related cysteine protease, Cysteine protease CPP32, Procaspase3, Protein Yama) is encoded by the CASP3 (also known as CPP32, CPP32B) gene (Gene ID 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is initially produced with a propeptide sequence (a.a. 1-9), the removal of which yields the 268 a.a. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (a.a. 10-175) fragment and the p12 (a.a. 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (a.a. 29-175) subunit. The p17 and p12 dimerizes and forms the active caspase-3 enzyme. Caspase-3 plays a central role in the sequential activation of caspases during the execution phase of cell apoptosis. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Specifications

• Antigen: Caspase 3
• Classification: Polyclonal
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: CASP3, CPP32
• Immunogen: KLH-conjugated linear peptide corresponding to human Caspase 3 near the C-terminus.
• Quantity: 50 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_004337
• Target Species: Human, Mouse

• Applications: Immunocytochemistry, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P42574
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Expected to react with pro-caspase 3, the uncleaved/inactive caspase 3 (32kDa), and the p12 (12kDa), but not the p17 (17kDa) subunit of cleaved/active caspase 3.
• Content And Storage: 2°C to 8°C for one year

For Research Use Only