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CD107a Rat anti-Mouse, Brilliant Violet 421, Clone: 1D4B, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564347

Catalog No. BDB564347

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The 1D4B antibody recognizes CD107a (Lysosome-Associated Membrane Protein 1, LAMP-1), one of the two major glycoproteins in lysosome membranes which are useful markers to distinguish lysosomes from other organelles. Mouse CD107a consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. Several lines of evidence suggest that cell-surface mouse and human LAMP-1 glycoproteins, the expression of which may correlate with malignant transformation, participate in intercellular adhesion and adhesion to extracellular matrix.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.



Brilliant Violet 421
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgG2a, κ
Flow Cytometry, Immunocytochemistry
LAMP-1; LGP-120; LGP-A; P2B
Affinity Purified
Plasma membrane fraction of mouse embryo NIH 3T3 cell line
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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