CD11b Rat anti-Mouse, APC, Clone: M1/70, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 553312

Catalog No. BDB553312

Description

The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or α;M) . CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, α;Mβ;2), also known as complement receptor 3 (CR3) . Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.

Specifications

• Antigen: CD11b
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: M1/70
• Conjugate: APC
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Human, Mouse
• Isotype: IgG2b κ

For Research Use Only.