CD11b Rat anti-Mouse, PE, Clone: M1/70, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 557397

Catalog No. BDB557397

Description

The M1/70 antibody reacts with the 170kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β2 integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: M1/70
Isotype: IgG2b, κ
Cross Reactivity: Mouse

Flow Cytometry

Specifications

• Antigen: CD11b
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: M1/70
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse Splenic Cells
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Human, Mouse
• Isotype: IgG2b κ

For Research Use Only.