CD121a Rat anti-Mouse, NA/LE, Unlabeled, Clone: 35F5, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 553693

Catalog No. BDB553693

Description

The 35F5 monoclonal antibody specifically binds to the Type I Interleukin-1 Receptor (IL-1 RI) which is also known as CD121a. CD121a is expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80 kDa type-I transmembrane glycoprotein, a member of the Ig superfamily that mediates the inflammatory responses of leucocytes to IL-1. The 35F5 antibody is capable of blocking the binding of IL-1α, IL-1β, and IL-1 receptor antagonist to the Type I IL-1 Receptor. The 35F5 mAb is highly specific for the Type I IL-1 Receptor and does not react with the Type II IL-1 Receptor (CD121b) which is present on mouse macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.

Specifications

• Antigen: CD121a
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: Il1r1; IL-1 Receptor Type I/p80; IL-1 R alpha; IL-1 Rα; IL-1 RI
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: 35F5
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ

For Research Use Only.