CD137 Rat anti-Mouse, Biotin, Clone: 1AH2, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 558974

Catalog No. BDB558974

Description

The 1AH2 clone (subclone of 53A2) has been reported to react with CD137, a member of the TNFR/NGFR superfamily. The expression of CD137 has been reported to be detectable from day 3 and peaks around day 6 after activation with ConA, PMA plus ionomycin, or immobilized anti-CD3e (Cat. No. 553058). Monomers, dimers, or tetramers of the 4-1BB antigen are expressed, upon activation, on the surface of splenic T lymphocytes, thymocytes, intestinal intraepithelial T lymphocytes (IEL), and some T cell lines and clones. While stimulating T cells by IL-2, IL-4, or anti-CD28 alone does not result in the expression of CD137; addition of IL-2, IL-4, anti-CD28, or syngeneic accessory cells to splenic T cells stimulated via TCR/CD3 can result in a high level of CD137 expression. CD137 has also been reported to be observed on IL-2 activated NK cells, but not on freshly isolated NK cells. It has been demonstrated that 4-1BB physically associates with p56 [lck]through a Cys-Arg-Cys-Pro binding site in its cytoplasmic domain; the same motif in the cytoplasmic tail of the CD4 and CD8a molecules is responsible for association with p56 [lck]. A signaling function for the CD137 molecule in mouse T cells is indicated by reports in which cross-linking of CD137 with 1AH2 mAb resulted in enhanced proliferation of CD3e-activated splenic T cells and IEL and in enhanced cytolytic activity of IEL in response to immobilized anti-CD3e. In addition to extracellular matrix proteins which bind to CD137, a 97-kDa dimer of the TNF/NGF superfamily has been reported to be a ligand for 4-1BB (4-1BBL). This molecule has been detected on Con A-activated T cells, LPS-activated macrophages, and anti-µ-activated splenic B cells. Interaction between T and B cells through 4-1BB/4-1BBL is reported to play a role in antigen presentation, further supporting a costimulatory role for CD137 in the immune response of T lymphocytes.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD137
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: 4-1BB, Ly-63
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 1AH2
• Conjugate: Biotin
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Recombinant mouse 4-1BB
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ