CD138 Rat anti-Mouse, Brilliant Violet 650, Clone: 281-2, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 564068

Catalog No. BDB564068

Description

The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

• Antigen: CD138
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 281-2
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: NAMRU mouse mammary gland epithelial cell line NMuMG
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.