CD14 Rat anti-Mouse, NA/LE, Unlabeled, Clone: 4C1/CD14, BD

Catalog No.	Quantity
BDB557896	0.5 mg

Catalog No. BDB557896 Supplier BD Biosciences Supplier No. 557896

Description

Rat Monoclonal Antibody

The 4C1/CD14 antibody reacts with CD14, a ∽55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein. Flow cytometric analysis demonstrates that 4C1/CD14 antibody stains CD14 transfectants, the monocyte/macrophage cell line RAW 264.7 peritoneal exudate neutrophils and macrophages, and cultured bone marrow-derived macrophages, but not undifferentiated bone marrow cells, splenocytes, or peripheral blood leukocytes. This staining pattern is similar to that of the alternate anti-mouse CD14 mAb rmC5-3, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils. Therefore, data suggest that CD14 expression by leukocyte populations may differ in mice and humans. CD14 is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein). After binding to LPS-LPB, CD14 associates with integral membrane proteins (such as TLR4-MD-2) which mediate signal transduction. It is involved in the development of endotoxic shock, innate immunity, inflammatory responses, and LPS-stimulated bone resorption. Unlike mAb rmC5-3, the 4C1/CD14 antibody blocks the binding of LPS to CD14.

Specifications

• Antigen: CD14
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 4C1/CD14
• Concentration: 1.0mg/mL
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Host Species: Rat
• Immunogen: Mouse monocyte/macrophage cell line
• Purification Method: Affinity Purified
• Quantity: 0.5 mg
• Regulatory Status: RUO
• Research Discipline: Immunology
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C
• Isotype: IgG2b κ

For Research Use Only.