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CD14 Mouse anti-Human, BD IMag-DM, Clone: MΦP9, BD

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Catalog No. BDB557769 Supplier BD Biosciences Supplier No. 557769
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Description

Mouse Monoclonal Antibody

BD IMag™ Anti-Human CD14 Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD14-bearing leukocytes using the BD IMagnet™. CD14 has been reported to be expressed at high levels on peripheral blood monocytes. Additionally, it is found on interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. BD IMag™ Anti-Human CD14 magnetic particles have been reported to effectively separate the CD14-bearing cells of rhesus macaque blood.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-human CD14 Magnetic Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. no. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD14
Applications Flow Cytometry
Classification Monoclonal
Clone MΦP9
Conjugate BD IMag-DM
Description LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Monocytes
Purification Method Affinity Purified
Quantity 1 x 109 Cells
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Includes Anti-Human CD14 Magnetic Particles, Mouse (BALB/c) IgG2b, κ (5mL)
Target Species Human, Rhesus Monkey
Content And Storage Store undiluted at 4°C
Isotype IgG2b κ
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For Research Use Only.

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