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Description
The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
Specifications
Specifications
| Antigen | CD14 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | M5E2 |
| Conjugate | Violet Fluor 450 (V450) |
| Description | LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein |
| Formulation | Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Human CD14 Protein |
| Purification Method | Affinity Purified |
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