CD140b (pY771) Mouse, Unlabeled, Clone: J23-618, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558361

Catalog No. BDB558361

Description

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchy mal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding indu ces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae , compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ.

The J23-618 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b . pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras .

Specifications

• Antigen: CD140b (pY771)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: CD140b (pY771)
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: J23-618
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human PDGFRβ Peptide
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG2a κ