CD140b (pY771) Mouse, PE, Clone: J23-1044, BD

Catalog No.	Quantity
BDB558426	50 Tests

Catalog No. BDB558426 Supplier BD Biosciences Supplier No. 558426

Description

Mouse Monoclonal Antibody

Platelet-derived growth f actor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of t he receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ.

The J23-1044 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b. pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.

Specifications

• Antigen: CD140b (pY771)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: J23-1044
• Conjugate: PE
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Phosphorylated Human PDGFRβ Peptide
• Quantity: 50 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ