CD15 Mouse anti-Human, BB515, Clone: HI98, BD

Catalog No.	Quantity
BDB565236	100 Tests

Catalog No. BDB565236 Supplier BD Biosciences Supplier No. 565236

Description

Mouse Monoclonal Antibody

The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X. This structure is found on a variety of cell surface glycolipids and glycoproteins. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment. Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD15
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HI98
• Conjugate: Brilliant Blue 515 (BB515)
• Description: Lewis X; Le-X; X-Hapten; SSEA-1; 3-FAL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Host Species: Mouse
• Immunogen: Human Mononuclear Cells
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgM κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov