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CD15 Mouse anti-Human, PE-CF594, Clone: HI98, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 562463
Description
The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X. This structure is found on a variety of cell surface glycolipids and glycoproteins. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment. Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications
CD15 | |
Monoclonal | |
PE-CF594 | |
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. | |
Human Mononuclear Cells | |
100 Tests | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
HI98 | |
Lewis X; Le-X; X-Hapten; SSEA-1; 3-FAL | |
Mouse | |
Affinity Purified | |
RUO | |
Human | |
IgM κ |
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