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CD15 Mouse anti-Human, PE-CF594, Clone: HI98, BD

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Description

Mouse Monoclonal Antibody

The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X. This structure is found on a variety of cell surface glycolipids and glycoproteins. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment. Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD15
Applications Flow Cytometry
Classification Monoclonal
Clone HI98
Conjugate PE-CF594
Description Lewis X; Le-X; X-Hapten; SSEA-1; 3-FAL
Formulation Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Mononuclear Cells
Purification Method Affinity Purified
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgM κ
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