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CD15 Mouse anti-Human, PE-CF594, Clone: W6D3, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 562372

Catalog No. BDB562372


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Description

Description

The W6D3 monoclonal antibody specifically binds to 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype; clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence staining and its binding can be blocked by clone HI98.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications

Specifications

CD15
W6D3
3-fucosyl-N-acetyllactosamine; 3-FAL
Affinity Purified
IgG1, κ
RUO
Primary
Human
Flow Cytometry
PE-CF594
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Mouse
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
SDS
Documents
Product Certifications
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