CD15 Mouse anti-Human, V450, Clone: HI98 (also known as HIM1), BD

Catalog No.	Quantity
BDB561584	50 Tests

Catalog No. BDB561584 Supplier BD Biosciences Supplier No. 561584

Description

Mouse Monoclonal Antibody

The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X. This structure is found on a variety of cell surface glycolipids and glycoproteins. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment. Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Specifications

• Antigen: CD15
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HI98 (also known as HIM1)
• Conjugate: Violet Fluor 450 (V450)
• Description: Lewis X; Le-X; X-Hapten; SSEA-1; 3-FAL
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Mononuclear Cells
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgM κ