CD152 Mouse anti-Human, Biotin, Clone: BNI3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 555852

Catalog No. BDB555852

Description

The BNI3 monoclonal antibody specifically binds to the human cytolytic T lymphocyte-associated antigen, CTLA-4. CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports that indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a possible role of CTLA-4 in the regulation of B-cell response. Immobilized BNI3.1 antibody enhances T-cell proliferation induced by antibody-mediated crosslinking of CD3 and CD28. Recent studies have shown that CD152 can be expressed by regulatory T (Treg) cells. After cellular fixation and permeabilization, the BNI3 antibody can stain intracellular CD152 expressed in T cells including Treg cells. Clone BNI3.1 was studied in the VI Leukocyte Typing Workshop.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD152
• Classification: Monoclonal
• Conjugate: Biotin
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human CTLA4 Recombinant Protein
• Quantity: 100 Tests
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: BNI3
• Description: CTLA-4; AILIM; Cytotoxic T-lymphocyte protein 4
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.