CD16 Mouse anti-Human, Brilliant Violet 510, Clone: 3G8, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB563829	BD Biosciences 563829	25 Tests
BDB563830	BD Biosciences 563830	100 Tests

Description

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γRIIa and Fc γRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD16
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 510
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: FcRIII; Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; IGFR3

• Clone: 3G8
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Immunogen: Human polymorphonuclear leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.