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CD16/CD32 Rat anti-Mouse, V450, Clone: 2.4G2, BD

Catalog No. BDB560539 Shop All BD Biosciences Products
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BDB560539 50 μg
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Catalog No. BDB560539 Supplier BD Biosciences Supplier No. 560539

Description

Rat Monoclonal Antibody

The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII and FcγII receptors. It has also been reported to bind the FcγI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, receptors. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD16/CD32
Applications Flow Cytometry
Classification Monoclonal
Clone 2.4G2
Concentration 0.2mg/mL
Conjugate Violet Fluor 450 (V450)
Description FcγRIII/FcγRII; Fcgr3/Fcgr2
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Host Species Rat
Immunogen Mouse BALB/c Macrophage J774
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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