CD16 Mouse anti-Human, PE-CF594, Clone: 3G8, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB562320	BD Biosciences 562320	25 Tests
BDB562293	BD Biosciences 562293	100 Tests

Description

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red (eg 610/20-nm filter).

Specifications

• Antigen: CD16
• Applications: Flow Cytometry
• Conjugate: PE-CF594
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: FcRIII; Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; IGFR3

• Clone: 3G8
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Immunogen: Human polymorphonuclear leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.